A Streptococcal Enzyme That Acts Specifically upon Diphosphopyridine Nucleotide: Characterization of T H E Enzyme and Its Separation from St1leptolysin O* by Arthur
نویسندگان
چکیده
Concentrated and partially purified preparations of streptococcal supernates containing streptolysin O have been shown to inhibit selectively the oxidative metabolism of mitochondria procured from the myocardium of rabbits and guinea pigs, the findings pointing to an interference with the action of the coenzyme diphosphopyridine nucleotide (DPN) (1). Further studies have demonstrated that these streptococcal preparations contain an enzyme, not hitherto discerned in streptococcal cultures, that specifically destroys DPN. Some of the characteristics of this enzyme, and its separation from streptolysin O, will be described in detail herein.
منابع مشابه
A Streptococcal Enzyme That Acts Specifically upon Diphosphopyridine Nucleotide: Characterization of the Enzyme and Its Separation from Streptolysin O
All enzyme that destroys DPN has been found in fractionated and highly concentrated streptococcal preparations that also contain streptolysin O. The enzyme-streptococcal DPNase-was shown by electrophoretic separation and by other means to be distinct from streptolysin O. It is non-dialyzable, heat-labile, and has optimal activity in the pH range 7.2 to 7.8. The enzyme has a high degree of speci...
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